Financing: The study was carried out within the framework of a state assignment, the research topic: “Development of a drug candidate with predominantly central vasodilatory action for the treatment of cerebrovascular pathologies”, state registration number — 124020900020-4.

Author contribution: Popov NS — development of the bioanalytical method, conducting pharmacokinetic studies, manuscript preparation; Gavrilenko DA — conducting pharmacokinetic studies, manuscript preparation; Baranov MS — synthesis of sydnonimine group compounds, manuscript preparation; Balabanyan VYu — defining the aim, developing the study design, manuscript preparation; Kaurova DE — literature review, manuscript preparation; Myasnyanko IN — synthesis of sydnonimine group compounds, manuscript preparation. All authors made an equal contribution to the preparation of the publication and confirm that their authorship meets the international ICMJE criteria, Ivanov DS — synthesis of compounds of the sydnone imine group, preparation of the manuscript.

Compliance with ethical standards: The study was approved by the Ethics Committee of Tver State Medical University of the Ministry of Health of Russia (Protocol No. 5 dated June 19, 2024). All experiments were performed in accordance with the Rules of Laboratory Practice (Order of the Ministry of Health of Russia No. 708n dated August 23, 2010, Directive 2010/63/EU of the European Parliament and of the Council on the protection of vertebrate animals used for scientific purposes).